Regulatory
Part:BBa_K3190002:Design
Designed by: Ojas Tulsyan Group: iGEM19_UCopenhagen (2019-10-12)
pCCW12: Yeast strong constitutive promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 298
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 298
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 298
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 298
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 298
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This promoter is used in our 5 constructs with receptors (GPER, GPER-sfGFP, XLHCGR, XLHCGR-sfGFP, Hu-LHCGR, Hu-LHCGR-sfGFP), which is used for genomic integration of the receptor genes in our modular system.
Source
S. cerevisiae